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KMID : 1059519880320020135
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Journal of the Korean Chemical Society
1988 Volume.32 No. 2 p.135 ~ p.143
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A Study of the Retention Mechanism of the Monosubstituted Benzenes in Reversed-phase Liquid Chromatography (II)
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Lee Dai-Woon
Choi Yong-Wook
Lee Won
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Abstract
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The correlations between chromatographic parameters of monosubstituted benzenes and several physical parameters in reversed phase liquid chromatography were studied. The relationships between retention data and polarity index were investigated by plotting log(log k'S/k'B) vs. P'S/P'B which were relative retention and relative polarity index of monosubstituted benzenes with respect to benzene, respectively. The linear relationship between relative retention and polarity index was observed for the monosubstituted benzenes having polar group, while in case of those having nonpolar group, the good linearity was observed by combination with relative molecular weight i.e. (P'S/P'B)/(MWS/MWB). Multivariant regression analysis, a(P'S/P'B) + b(MWS/MWB) + c did not give significantly better correlations compared to single variant analysis, a[(P'S/P'B)/(MWS/MWB)] + c, but multiple stepwise regression analysis was recommended when several physical parameters simultaneously were chosen. The best correlation between retention data for monosubstituted benzenes taken from the literature and substituent constant(π), derived from hydrophobic parameter and the first order molecular connectivity index(χν), was established for methanol/water mobile phase system. The larger the surface coverage of the stationary phase, the higher was the correlation coefficient between these two parameters and retention data.
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